Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Email promega technical services if you have uestions on use of this system. Email promega technical services if you have questions on use of this system. Powerplexr 21 system for use on the applied biosystemsr. The powerplex 16 hs system is more tolerant of pcr inhibitors than competing str systems and the previous version of the powerplex 16 system, increasing your success rate and reducing the need for reamplification. Pdf the powerplex 16 bio multiplex short tandem repeat str system contains the codis loci fga, tpox. This manual contains protocols for use of the powerplex16 system with the perkinelmer model 480 and geneamppcr system 9600, 9700 and 2400 thermal cyclers in addition to. Ystr markers are found on the nonrecombining region of the y chromosome nry and. Summary of changes the following changes were made to the 6 16. Instructions for use of products e2920, e2940, e2980. The system allows the coamplification and threecolor detection of sixteen loci fifteen str loci and amelogenin. This paper describes the developmental validation study performed on the powerplex esi 16 european standard investigator 16 and the powerplex esi 17 systems. Spectrum compact ce system promega france pdf catalogs.
Technical manual revised 516 tmd023 powerplex esx 16 system instruc ons for use of products dc6710 and dc6711. Powerplex 16 hs system technical manual, tmd022 promega. Powerplexr esx 17 fast system for use on the applied. Amplification kits globalfiler and globalfiler iqc pcr. The powerplex 16 system provides all materials necessary to amplify str regions of human genomic dna except for amplitaq gold dna polymerase. Refer to the applied biosystems 35003500xl genetic analyzer user guide for the. For an explanation of the proper usage and effects of these settings, refer to the applied biosystems user bulletin titled. Page 3 the powerplex 16 system provides all materials necessary to amplify str regions of human genomic dna except for amplitaq golddna polymerase. Email promega technical services if you have questions on use of this. Powerplex esi 16 fast system for use on the applied. Pdf validation and implementation of the powerplex 16 bio. Geneprintr fluorescent str systems technical manual, tmd006.
The powerplex esx 16 system provides all materials necessary to amplify str regions of human genomic dna, including hotstart taq dna polymerase, which is a component of the powerplex esx 5x master mix. This rare phenomenon has been observed with the promega powerplex esi systems at the loci d12s391, d2s441 and d1s1656. Internal validation of powerplex 16 hs with the applied biosystems. For more information on the iqc markers, see chapter 5, assess the pcr reaction with the internal quality control system.
The powerplex esi 16 system is compatible with the abi prism 310, 3100 and 3100avant genetic analyzers and applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers. Powerplex 16 system or identifiler kit data not shown. At 300ng of humic acid only the penta d and penta e loci dropped out with powerplex 16, whereas the d7s820, csf1po. The powerplexesi 17 fast system provides all materials necessary to amplify str regions of human genomic dna, including a hotstart thermostable dna polymerase, which is a component of the powerplexesiesx fast 5x master mix. Development and validation of a rapid pcr method for the. This manual contains protocols for use of the powerplex 16 system with the perkinelmer model 480 and geneamp pcr system 9600, 9700 and 2400 thermal cyclers in addition to. The molecular weight of the amplicons from each of the 16 pools was estimated by taking the product of 650 and their base pair length. Developmental validation of the powerplex esi 1617 fast.
The system combines common and informative loci to overcome typical challenges faced by laboratories, including sample inhibitors, low levels of input dna, degradation, and mixtures. The powerplex 16 hs system allows coamplification and threecolor detection of. Because it is a male dna, the control dna is appropriate for use with all powerplex systems. This manual contains protocols for use of the powerplex 16 system with the perkinelmer model 480 and geneamppcr system 9600, 9700 and 2400 thermal cyclers in addition to protocols to separate amplified products and detect separated material.
Dna technical leader powerplex fusion sample preparation. Project muse how much dna is lost measuring dna loss of. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa. A decrease in the amplification time would allow for increased laboratory throughput, which may help reduce backlog when is due to limited cycling. Powerplexr 5c matrix standards, 310 technical manual. Analyze samples as described in the users manual for the abi prism 3100 or. Internal lane standard resolved on an applied biosystems 3xl genetic analyzer.
The powerplex 16 system is a multiplex str system for use in dna typing, including paternity testing, forensic dna analysis, human identity testing and tissue culture strain identification. Visit the web site to verify that you are using the most current version of this technical manual. This dna purification chapter addresses general information on the basics of dna isolation, plasmid growth and dna quantitation as well as how purification by silica can help increase your productivity so you spend less time purifying dna and more time developing experiments and analyzing data. Tmd035 revised 616 the powerplex y23 system provides all materials necessary to amplify ystr regions of human genomic dna, including a hotstart dna polymerase, which is a component of the powerplex y23 5x master mix. We have 2 toro powerplex series manuals available for free pdf download.
This manual contains a protocol for use of the powerplexesi 17 fast system with the geneamp. All technical literature is available on the internet at. Powerplex 16 hs system protocol promega corporation. Powerplexr 4c matrix standard technical manual tmd048.
Powerplex 16 system technical manual, tmd012 promega. Forensic biology protocols for forensic str analysismanual appendix for powerplex fusion status. View online or download everfocus powerplex edr1640 instruction manual. Dualluciferase reporter assay system protocol promega. A protocol for coamplification and threecolor detection of sixteen loci fifteen. Genetics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Full profiles were detected in the presence of up to 250ng of humic acid per 25l amplification reaction with powerplex 16 but only up to 200ng for the identifiler kit. Promega is replacing the 9947a dna and 9948 male dna provided in powerplex systems with 2800m control dna beginning in 2012. Powerplexr 18d system for use on the applied biosystemsr. The internal lane standard, wen internal lane standard 500 ess, is designed and optimized specifically for this system. Direct amplification from buccal and blood samples preserved.
Refer to the applied biosystems 35003500xl genetic analyzer user guide for the instrument maintenance schedule and instructions to install the capillary array. By using the speedstar hs polymerase takara bio, otsu, japan in combination with the veriti applied biosystems, foster city, ca, usa rapid thermal cycler, the amplification time for the powerplex 16 hs promega, madison, wi, usa kit was reduced by 66 % 1 h. Decreasing amounts of template were amplified using the powerplex 16 hs system. Email promega technical services if you have uestions on use of this system promega corporation 2800 woods hollow road madison. Internal lane standard 60bp 600bp, rox product insert for. Feb 11, 2015 promega corporation announces the release of the powerplex fusion 6c system, a 6color, 27locus multiplex.
An omniswab buccal sample was pretreated with swabsolution reagent as recommended in the swabsolution kit technical manual tmd037. This manual contains protocols for use of the powerplex fusion 6c system with the geneamp pcr system 9. Promega corporation 2800 woods hollow road madison. The powerplex 16 hs system provides all of the materials necessary for amplification of these loci, including hotstart taq dna polymerase, and is compatible with the abi prism 310, 3100 and 3100avant and applied biosystems 3 and 3xl genetic analyzers. This manual contains a protocol for use of the powerplex esx 16 system with the geneamppcr system 9700 and. The powerplex fusion system provides all of the materials needed for coamplification and fivecolor fluorescent detection of 24 loci 23 str loci and amelogenin, including the codis. Promega powerplex fusion 6c system the scientist magazine. Prepfiler express tm and prepfiler express bta tm forensic dna extraction kits user guide.
This new control dna is a singlesource male human genomic dna purified from blood. A protocol for the powerplex esi 16 fast system, for coamplification and fourcolor fluorescent detection of new loci recommended by the european network of forensic science institutes. The powerplex fusion 6c system provides all materials necessary to amplify str regions of human genomic dna, including a hotstart thermostable dna polymerase, which is a component of the powerplex fusion 6c 5x master mix. Electropherogram of the powerplex fusion 6c system allelic ladder run on prototype spectrum compact ce system. The powerplex 16 hs system master mix is more tolerant of pcr inhibitors than competitive str systems. Compatible with products for fragment analysis including promega powerplex 16 system contents. The powerplex 21 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block. We have 5 everfocus powerplex edr1640 manuals available for free pdf download. Developmental validation of the powerplex esi 1617 fast and.
The powerplex esi 17 pro system provides all materials necessary to amplify str regions of human genomic dna, including hotstart taq dna polymerase, which is a component of the powerplex esi 5x master mix. Description of artefacts in the powerplex y23 system. A pilot study looking at sexual assault cases where no spermatozoa were present was performed using the powerplex y23 system samples with a large excess of female dna present generated artefact peaks. In response to the enfsi and ednap groups call for new str multiplexes for europe, promega developed a suite of four new dna profiling kits. Installation procedures and new features for genemapper id software 3.
Refer to the 3500 series data collection software hid user manual for the. Quick 2prtc thermal cycling use of the powerplex 21 system to amplify dna from storage card punches instructions for use of products dc8902 and dc8942. Amplified products were separated on an applied biosystems 3xl genetic analyzer 3kv, 5second injection. The powerplex esi 16 fast, esi 17 fast, esx 16 fast, and esx 17 fast. The dualglo luciferase assay system is designed to allow highthroughput analysis of mammalian cells containing genes for firefly and renilla luciferases, grown in 96 or 384well plates. For steps 11 and 12, see the genemapperz id user s manual for more information. Where the promega 9948 male dna is heterozygous at 11 of the powerplex 16 system markers, we used the mean length of the amplicon sizes. The system contains the loci penta e, d18s51, d21s11, th01, d3s58, fga, tpox, d8s1179, vwa, amelogenin, penta d, csf1po, d16s539, d7s820, ds317 and d5s818. This manual contains a protocol for use of the powerplex esi 17 pro system with the geneamp pcr system 9700 and 2720. Electropherogram of 1ng of 2800m control dna analyzed with powerplex fusion 6c system run on prototype spectrum compact ce system.
The authors demonstrate the effectiveness of the powerplex 16 hs system for direct amplification of dna from buccal and blood samples preserved on fta and protein saver 903 cards. The following changes were made to the 5 16 revision of this. Forensic biology protocols for forensic str analysisamplification using the power plex fusion system status. The primer set and the allelic ladder in the globalfiler kit are different.
Validation and implementation of the powerplex 16 bio system str multiplex for forensic casework. This manual contains a protocol for use of the powerplex esx 17 fast system with the geneamp pcr system 9700 and veriti 96well thermal cycler in addition to protocols to separate amplified products and detect. In addition to the loci listed above, the globalfiler iqc pcr amplification kit amplifies 2 internal quality control iqc markers. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. The powerplex16 system provides all materials necessary to amplify str regions of human genomic dna except for amplitaq golddna polymerase. Currently, the amplification step of most forensic dna profiling systems takes 34 h to complete. Nov 01, 2014 read developmental validation of the powerplex esi 1617 fast and powerplex esx 1617 fast systems, forensic science international. All primer pairs, with the exception of the se33 primer pairs, are identical in both systems. In some cases, the data analysis parameters in the genemapper software can be adjusted, in accordance with the applied biosystems genemapper user manual, to analyse the data.
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